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How to: Create a kinetic assay on the VICTOR injecting between groups of measurements

Document ID: DQ319
Relevant to following products: WorkOut 2.5
Document description: How to: Create a kinetic assay on the VICTOR injecting between groups of measurements



Unfortunately the VICTOR Manager Software cannot be setup to take kinetic measurements (with plate repeats) that injects in between groups of cycles. For example:

1. Read the samples (96 microplate) for 5 min (repeat measurements every 5 sec.)
2. Inject first compound , Read for 10 min repeat measurements every 5 sec)
3. Inject second compound , Read for 5 min (repeat measurements every 5 sec.)

By using WorkOut this assay can be configured. Basically create 3 online WorkOut protocols for each measurement phase and then a 4th protocol to import and combine the data. To get started create these protocols as Protocol 1, Protocol 2 and Protocol 3 and setup the measurements as required.

The combination of all three sets of data is achieved by exporting and appending the results to a text file. Thus, to each of the 3 measurement protocols add a Text Export transform. To each, set the target filename to be the same file, such as

C:\Documents and Settings\UserName\My Documents\My Assays\VictorRun.txt



Add this same transform to the Protocol 2 and Protocol 3, the difference being that the Append to existing file option is ticked in the 2nd and 3rd (but not in the first).

A starting point for the 4th protocol is provided here, this will import all the data from the target file into a single WorkOut file. Please modify this protocol as required for further analysis (such as Kinetic Join). You can test this protocol out by opening it and selecting the accompanying example file.

To automate this whole process you can use a batch file. Create this in notepad. The file should look like this (though change the paths/directories accordingly):

"c:\Program Files\Dazdaq\WorkOut 2.5\MRunRes.exe" /run "C:\Documents and Settings\UserName\My Documents\My Assays\Protocols\Protocol 1.apr" /exit
"c:\Program Files\Dazdaq\WorkOut 2.5\MRunRes.exe" /run "C:\Documents and Settings\UserName\My Documents\My Assays\Protocols\Protocol 2.apr" /exit
"c:\Program Files\Dazdaq\WorkOut 2.5\MRunRes.exe" /run "C:\Documents and Settings\UserName\My Documents\My Assays\Protocols\Protocol 3.apr" /exit
"c:\Program Files\Dazdaq\WorkOut 2.5\MRunRes.exe" "C:\Documents and Settings\UserName\My Documents\My Assays\Protocols\Protocol 4.apr" "C:\Documents and Settings\UserName\My Documents\My Assays\VictorRun.txt"

Make sure you include the quotes as used here. Also when saving this in notepad be sure to enter the name with quotes around it e.g. “VICTOR Run.bat” otherwise notepad won’t save it as a .bat file. Now when you run this .bat file the whole process will be performed automatically.





Related articles:
DQ311: How to: Combine data from two separate runs into one results file
How to: Combine data from two separate runs into one results file





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