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How To: Setup an ELISA protocol with dual measurements?

Document ID: DQ245
Relevant to following products: WorkOut 2.0 Manta
Document description: How To: Setup an ELISA protocol with dual measurements?

To create an ELISA protocol using dual measurements (for example a measuments at 450nm followed by a measurement at 595nm):  

From the main Organiser screen, select Create a new protocol, then select the Quantitative Wizard. The wizard will take you through the steps of setting up the protocol.

On the Data Acquisition step of the wizard, press Create… to setup your dual measurements (such as at 450nm and 495nm). Continue through the Wizard, specifying your microplate layout and other analysis parameters. On completion of the Wizard run the protocol for the first time.

By using 2 measurements the wizard will automatically setup a "Dual Matrix Expression" transform, this performs the subtraction.

Related articles:
DQ227: How To: Edit a transform
How To: Edit a transform

DQ013: What is a transform?
Definition: Transform

DQ043: How do I create a typical online assay protocol?
How to: Take measurements with an instrument for a typical assay that will be used routinely

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