Document ID: DQ235 Relevant to following products: WorkOut 2.0 Manta Document description: How To: Access the Kinetic Fit parameters

A Kinetic Fit transform fits a curve to the kinetic plot of each well. The resulting output for each well is a kinetic plot of the fit through the processed data.

There is probably some parameter of the fit that you would like to analyse. For example, with a linear regression Kinetic Fit you may be interested in the slope of the reaction in each well.

The fit parameters are available as well output variables and these can be used in any of the software’s expressions. For example, to access the slope of a Kinetic Fit transform using linear regression:

Create a new transform Matrix Expression transform and enter

#1:m

The number after the # indicates the transform number (here the Kinetic Fit transform is the first transform) and the variable after the : is the fit parameter.

The fit parameters available will depend on your selected fit (see below).

Since these parameters are available wherever an expression can be entered you can use them as parameters to other transforms for flexible analysis, such as validation, auto-flag, setting range, etc.