How to: Find the difference between two sample types on a plate.Document ID: DQ198
Relevant to following products: Manta WorkOut 2.0
Document description: How to: Find the difference between two sample types on a plate.
It is useful to be able to calculate the difference between one sample type and another.or example, an assay has two types of samples (X and Y) in triplicate with a blank group. A blank correction is performed on all raw measurements. The difference between the each Y group and its associated X group is to be calcaulted.
Use the Generic Wizard to get started. On the microplate layout step press Create to setup the microplate layout. This should include the blank and two groups, such as Unknown X and Unknown Y (use the Edit Types button in the microplate layout editor). Complete the Wizard and run the assay for the first time, then press Edit Transforms, remove the %CV transform. Now create a new “Matrix Expression” transform, under the Input Matrix tab, press Advanced and select Blank and UnkownY (so that the Y-X calculation is only performed on the UnknownX wells). Under the Expressions tab enter the expression:
UnknownY(n) – x
This calculates the Y-X. It does not matter that it does it on a well-by-well basis as the average is calculated in the table.
Click here to download the example files.