How can I set up a ratio calculation using data read from two separate plates?
Document ID: DQ180 Relevant to following products: WorkOut 2.0 Manta Document description: How To: Set up a ratio calculation using data read from two separate plates.
Use the Generic Wizard and proceed to set up your measurements. On the Microplate Layouts step press the Advanced… button and ensure that “Create Multiple Microplate Layouts” is ticked. Press Create… on the Microplate Layouts step. Use the microplate layout to set up two microplate layouts. Assuming both layouts are the same, first set up the first plate layout and then use the Repeat Last button to create the second. Press Save and Close to return to the wizard and complete the wizard, then take the measurements for the first time.
Once you have the measurements press the Edit Transforms button; if necessary remove the %CV transform. Press Create… and select Matrix Expression transform and under the Expression tab enter:
g(n)..2 / g(n)..1
This divides the mean of each group on the second plate by the mean of each group on the first plate. Return to the main window and press Recalculate. The table of results will show these results next to the first plate of data.