How To: Setup Chromo-Lal Limulus Amebocyte Lysate assay
Document ID: DQ151 Relevant to following products: WorkOut 2.0 Manta Document description: How To: Setup Chromo-Lal Limulus Amebocyte Lysate assay
Use the Generic Wizard to get started, setup the measurements or import the data and setup the microplate layout containing your Standards and Neg Control as described.
When you have ran the protocol for the first time and have the raw data in WorkOut, press Edit Transforms and remove any transforms added by the Wizard. Now Create a new transform and select Delta X for Delta Y – the help text displayed explains what it will do. As it is the time they are interested in ensure that under the Kinetic Data tab the X axis is set to time. Under the Difference tab enter the OD value (i.e. between 0.03 and 0.2). Under the Output Matrix tab, enter “Onset Time”
Next, create a Standard Curve Fit transform using the “Onset Time” as the input matrix, setup the Concentrations as described and select Log axes.