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I want to run a Quantitative assay with the standards measured on every plate, but each time I run the assay I change the number of samples and hence the number of plates required.

Document ID: DQ119
Relevant to following products: WorkOut 2.0 Manta
Document description: How To: Create a Quantitative assay which will use a variable number of samples and plates with the concentrations calculated using each plate’s own standards.



Follow these instructions to create a Quantitative assay which uses the same microplate layout on every plate that is read. Here the standards occur on every read plate; the number of Unknowns can be adjusted prior to each read with the software calculating the number of plates required.

The calculations will be performed on a plate by plate basis, i.e. a curve fit will be made for each plate and the concentrations will be calculated using a “local” curve fit.

Please note build 294 or later is required (contact Dazdaq Solutions if you require an update).

From the Organiser select Create a new protocol. Select the Quantitative Wizard. Setup the Data Acquisition as required. On the Microplate Layouts step, press the Advanced button and ensure that the Create Multiple Plate Microplate Layouts control is ticked. Returning to the Microplate Layouts step, press Create.

From the Microplate Layout Editor: double click on Plate Layout 1 and setup the Microplate Layout as required. This will include your standards, unknowns and optionally other groups such as blanks and controls. Setup the Microplate Layout to include the maximum number of Unknowns that will be read on one plate (when the assay is run the number of Unknowns can be adjusted and the required number of plates calculated for you). Press Save and Close to exit the Microplate Layout editor and return to the wizard. Continue through the wizard entering the settings for your Quantitative assay and launch the protocol ready to run.

Each time you run the protocol you can press the Start button as normal to start the measurements with the default settings, i.e. reading one whole plate. Alternatively, you can adjust the number of Unknowns and hence the number of plates by using the Scrubber Wizard.

To adjust the number of Unknowns, press the Scrubber Wizard icon (this is on the left of the Sample IDs button and appears as an eraser scrubbing wells). Press Next on the Wizard’s welcome screen. On the main Scrubber Wizard step you can enter the number of Unknowns required on this run of the assay, use the mouse to select the Unknowns group to modify and enter the number of Unknowns required, then press Enter. The Wizard calculates the number of plates required to read the required number of Unknowns and updates the Microplate view on the left to show the plates to be read. The number entered can be any number; it is likely that the last plate read will only be partially filled with unknowns. When you are happy with your selection continue through the wizard and start the measurements.

After measurements are completed you can view the fit on each plate under then Analysis tab, you can also overlay the fits from each plate.



Related articles:
DQ054: Can I use a standard curve from one plate on other plates read in the same batch?
Application Example: RIA on Multiple plates.

DQ120: I want to run a Quantitative assay with the standards measured in a different location on each plate read.
How To: Create a Quantitative assay with the standards in a different place on each plate and with the concentrations calculated using each plate’s own standards.

DQ101: How do I find which version of the software I am using?
How To: Find which version of the software is in use.





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