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How can I blank correct my samples?

Document ID: DQ058
Relevant to following products: Manta WorkOut 2.0
Document description: How To: Setup blank correction…



Each endpoint wizard will automatically set-up blank correction analysis for you based on the selected microplate layout.  If there are any blank wells on the microplate(s) then a blank correction transform is created by the wizard.
 
The different types of blank corrections available are as follows (with example microplate layouts - blank wells in yellow):
  • Single - where there is one blank group on the plate
  • One for one- each unknown has its own blank group
  • Random- any association between blanks and unknowns can be specified. This allows for any type of blank correction configuration, such as microplate layouts with: blanks in each corner of the plate, blanks in each half of the plate, random blanks, etc.

With Single and One for One the wizard will automatically set-up the blank correction analysis and no additional wizard step is displayed.
 
With Random an additional wizard step is displayed for specifying the random associations.
 
In all cases of blank correction the average of wells in a blank group is subtracted from associated wells.

More Details:
The Blank Correction transform works by associating wells with a blank group to correct.  Each well can be associated with one blank group. When the transform is evaluated each well has the mean of its associated blank group subtracted from it.
When the blank correction transform is created default associations are created depending on the microplate layout:
If there is one blank group on the plate then all wells are associated with that blank group.

  • If there is an equal number of blank groups and unknown groups then each unknown group is associated with the blank group of the same number, eg Unknown2 and Blank1, Unknown2 and Blank2, etc.
  • If the microplate layout does not fit into these two categories then no default associations are made; associations can be specified with the user interface as required.

Blank correction setup by the wizard is always performed plate by plate, that is a blank group can only be associated with wells on the same plate it was read.

Note: When reading multiple plates in your assay it is possible to setup blank correction using a blank group from a specific plate using functionality outside of the wizard.





Related articles:
DQ013: What is a transform?
Definition: Transform

DQ016: What is the Microplate Layout?
Definition: Microplate Layout

DQ040: What is the quickest way to take measurements now?
How to: Take measurements with an instrument now and setup some analysis afterwards…

DQ213: How To: Blank correct using blanks read on a different plate?
How To: Blank correct using blanks read on a different plate?





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