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Can I run assays across multiple plates?

Document ID: DQ046
Relevant to following products:  Manta 2.0 WorkOut 2.0
Document description: How to: Use a compatible stacker system to run assays across multiple microplates

To setup and launch a typical Assay Protocol:

  1. From the Organiser Welcome Screen select Create a new protocol.
  2. The Select New Assay Protocol Wizard screen will be displayed, select a Wizard which best describes the assay you are running (if you have further analysis requirements these can be added later).
  3. Press OK to start the Wizard.
  4. Press the Next button to step through the Wizard.
  5. On the Data Acquisition Method step press the Create... button to specify the measurements to perform in the instrument's own configuration window.
  6. On returning to the Wizard step press Next.
  7. On the Microplate Layouts, press the Advanced... button and ensure the Create Multiple Plate Microplate Layouts option is ticked. Press OK to return to the wizard.
  8. Press the Create... button to launch the Microplate Layout Editor and use this to define the layout of each microplate to use.
  9. Press the Save and Close button to return to the Wizard.
  10. Press Next to continue through the remaining steps of the Wizard specifying your assay parameters as you go.
  11. On the final Wizard step press the Finish button to launch the new Assay Protocol.

To take the measurements:

  1. Press the Start button on the horizontal toolbar in the main Run and Results window.
  2. As each new plate is automatically loaded and readings are taken they will appear on the screen.
  3. After the readings have been completed you can Review the Results.

To run the assay again:

  1. Press the New button in the main Run and Results window.
  2. Click OK to use the current Assay Protocol (if multiple versions have been created select which version to use - typically you would use the latest).
  3. Start the assay as described above.

To run the assay again at a later date:

  1. From the Organiser Welcome Screen select Run or open an existing protocol.

If you have further analysis requirements

  1. Press the Edit Transforms button in the main Run and Results window.
  2. Press the Create... button to create transforms as required.

Related articles:
DQ012: What is the difference between offline and online modes?
Definition: Online and Offline

DQ016: What is the Microplate Layout?
Definition: Microplate Layout

DQ054: Can I use a standard curve from one plate on other plates read in the same batch?
Application Example: RIA on Multiple plates.

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