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How do I create a typical online assay protocol?

Document ID: DQ043
Relevant to following products:  Manta 2.0 WorkOut 2.0
Document description: How to: Take measurements with an instrument for a typical assay that will be used routinely



To setup and launch a typical Assay Protocol:

  1. From the Organiser Welcome Screen select Create a new protocol.
  2. The Select New Assay Protocol Wizard screen will be displayed, select a Wizard which best describes the assay you are running (if you have further analysis requirements these can be added later).
  3. Press OK to start the Wizard.
  4. Press the Next button to step through the Wizard.
  5. On the Data Acquisition Method step press the Create... button to specify the measurements to perform in the instrument's own configuration window.
  6. On returning to the Wizard step press Next.
  7. On the Microplate Layouts step select an existing Microplate Layout or click Create... to specify the layout of your samples (you can change this later if required).
  8. Press Next to continue through the remaining steps of the Wizard specifying your assay parameters as you go.
  9. On the final Wizard step press the Finish button to launch the new Assay Protocol.

To take the measurements:

  1. Press the Start button on the horizontal toolbar in the main Run and Results window.
  2. After the readings have been completed you can Review the Results.

To run the assay again:

  1. Press the New button in the main Run and Results window.
  2. Click OK to use the current Assay Protocol (if multiple versions have been created select which version to use - typically you would use the latest)
  3. Start the assay as described above.

To run the assay again at a later date:

  1. From the Organiser Welcome Screen select Run or open an existing protocol.

If you have further analysis requirements:

  1. Press the Edit Transforms button in the main Run and Results window.
  2. Press the Create... button to create transforms as required.




Related articles:
DQ040: What is the quickest way to take measurements now?
How to: Take measurements with an instrument now and setup some analysis afterwards…

DQ014: What is an Assay Protocol?
Definition: Assay Protocol

DQ012: What is the difference between offline and online modes?
Definition: Online and Offline





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