Document ID: DQ024 Relevant to following products: Manta WorkOut 2.0 Document description: How is the MSE and SS of Cubic Spline calculated?

By definition the Cubic Spline goes
through all points therefore you may consider that the MSE should be 0.
However, as with the other fits, the software calculates the MSE of each
replicate point against the fit, rather than the mean of the replicates in
each standard group. The MSE of the Cubic Spline against the means would be
0 (because the spline goes through all mean points), however the MSE of the
replicate points is a more useful measure as it is a measure of the error in
the replicates.