Home     Contact     Sitemap
        
  Support

Can I modify my raw data based on its relation to a calibrator?

Document ID: DQ018
Relevant to following products: Manta WorkOut 2.0
Document description: How To: Modify raw data conditionally with a calibrator…



It is not possible to “modify raw data” however you can create a transform which produces output based using a condition on the raw data.
 
For example, in one assay each sample is compared against the measurement of a calibrator (in well A1), if the sample < the calibrator then the sample result should be set to the calibrator’s measurement.
 
To achieve this create a Matrix Expression Transform and enter the following expression:
 
((x < A1) * A1) + ((x > A1) * x) + ((x = A1) * A1)
 
This expression is evaluated in each well (x is substituted for the measurement of each well) and results in a new matrix where each sample with a measurement less that A1 is set to A1’s measurement. 
 
The expression uses Boolean logic and relies on the fact that logic is evaluated to 0 or 1.  The 3 branches of the expression must be mutually exclusive, ie. If the sample is <= A1 it is set to A1 otherwise the sample’s original value is preserved.
 
You can substitute A1 for a specific sample name if using replicates (such as Control1).



Related articles:
DQ013: What is a transform?
Definition: Transform

DQ032: How do I use the changes I have made to the result analysis in future runs of the assay?
How To: Extract a version of the protocol for use in the future…





 Search again

  Evaluate
  Download software now:

    WorkOut 2.5
    Manta
    Stingray
  Support
    Search Knowledge Base
    Browse Knowledge Base
    Submit a query