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What is the Microplate Layout?

Document ID: DQ016
Relevant to following products: Manta WorkOut 2.0
Document description: Definition: Microplate Layout

Microplate Layout

The Microplate Layout describes the positioning of the samples on the microplates associated with the test. The layout corresponds to how the physical microplates were or will be pipetted when the data is acquired.

The microplate layout is specified using group types and group numbers. All wells with the same sample type and group number are replicates and are considered as belonging to the same group.

For example, a Quantitative application may require a microplate layout with Standard, Blank, Unknown, Pos and Neg Control group types. There may be 8 Standard groups, 1 blank group, 1 Pos and 1 Neg Control, with the rest of the microplate filled with Unknown groups. If the assay uses duplicates then each group will occur in two wells. In this example wells A1 and A2 may be occupied by replicates of the group "Standard1".
Within Manta each group type is represented by a particular colour and each group number by a number in the well. 

Manta places no restriction on the positioning of wells within the layout - replicates do not need to be adjacent. However group numbering must start at 1 and ascend sequentially.

For Unknown group types Sample IDs can be specified. For assays running across multiple microplates, microplate layouts can be defined for microplate sequences.  The microplate layout can be modified after readings have been made if pipetting errors have been made.

Related articles:
DQ011: How can I run an HTS assay which reads more than one plate?
How To: Run an assay over multiple plates…

DQ206: How to: Create custom sample type
How to: Create custom sample type

DQ099: How Can Sample IDs be specified?
How To: Specify Sample IDs.

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