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 Stingray Features

32-bit Windows software
Can be used under Windows 95/98/NT/2000/XP

Context Sensitive Help
Press F1 or click a help button at any point whilst using Stingray for relevant Help information about the task at hand.

Multiple Document Interface
This means that any number of protocol, standard and reading files can be open at once. Windows can be arranged automatically using the standard tile and cascade commands.

Active Data Views
When data is changed in one view of a file, all other views of the data are modified instantly to reflect the changes made.

Drag And Drop Control Bars
Relevant control bars are displayed as and when required.

Short-Cut Keys

Experienced users can use key combinations to quickly perform common tasks.

Protocol/Results separation
Stingray splits assay use between two concepts, design and results. This approach allows an assay to be designed and re-used many times to run routine tests and provide a consistent reporting method. Isolating assay design from the end user also eliminates the possibility of accidental assay corruption. Stingray also provides a facility for changing the protocol file after readings have been made, if required.

Multiple raw data readings within one protocol

Acquire, access and combine multiple matrices of raw data using one or more connected machines within one protocol.

Interleaved Kinetics
Run a kinetic test reading each cycle at different instrument settings.

Assay Master
Guides the user through the maze of protocol design.

Variable Microplate Dimensions
Stingray can support any microplate dimensions.

Virtual Microplate

Use a virtual microplate to design template layouts that are directly portable between microplate readers and single tube readings.

Flexible Template Configuration
Use the mouse to quickly design any possible template layout with replicates and in any fill direction. Undo command to recover from mistakes.

Template Layout Printing
Print the template layout out in colour to remind protocol users how to fill a microplate, or to insert tubes.

Read Order Configuration
Use the mouse to configure the read order of wells in any possible order (instrument dependent).

Good Laboratory Practice (GLP)
Easily create data entry screen to appear before a protocol run to gather assay specific information. This data can be included in the report.

Automatic Flagging
Stingray can automatically flag wells which satisfy expression based conditions or automatically flag the well that is the furthest from the mean for all groups with a percentage CV greater than a specified value. All flagged items are logged and can be included in the final report.

Powerful Expression Based Transformations
Create data transformations using standard mathematical grammar on raw or calculated matrices, using references to wells, defined groups (e.g. sample1) or groups of groups (e.g. samplen).

Transformation Wizards
Quickly create transformations for common tasks: blank correction, curve fit, matrix difference, kinetic difference, etc.

Curve Fits
- Point to Point
- Cubic Spline
- Linear Regression on replicate points, with a specified validation correlation coefficient
- Polynomial Regression
- Four Parameter Fit for sigmoidal plots
- Flexible Graph Axes and Titles
- Logarithmic or Linear x and/or y axes.

Curve Fit data source from readings or archived standards file

Multiple standards on one plate

Ten kinetic reduction methods available

The number of regression points can be specified where appropriate.

Positive or negative maximum slope
Calculate reductions using the most positive or most negative slope.

Kinetic Fail options
Specify what happens if a reduction method fails (where thresholds are involved).

Flexible Validation Specification
Specify validations for raw or calculated matrices using standard mathematical expressions on defined groups or wells, including Boolean operators and statistical operations.

Flexible Cut-Off Specification
Specify Cut-Offs as expressions for raw or calculated matrices using standard mathematical expressions on defined groups or wells, including Boolean operators and statistical operations.

Numerical Formatting
Specify how to format numerical data (for display/reporting purposes only) using decimal places or significant figures.

Flexible Reporting specification
Choose which data to include in the report and in which order. Edit report headers and footers, use any installed Windows fonts. The report can include a table of results (by group), a matrix of results (by well), fitted graphs, any fixed text, readings notes, validation results, calculation log, automatic flagging results, list of flagged wells and 3D graphs. Report layout can be previewed before readings are taken.

Editing of the protocol after readings have been taken
Make modifications to a protocol after readings have been made (user privilege dependent).

Flexible runtime kinetics parameters
Specify kinetic interval, number of cycles or total time at run time or use protocol defaults.

Stop kinetic test at any point preserving readings already made

View kinetic data during readings
Kinetic data can be viewed as a matrix of graphs, individual zoomed graphs, cycle times or cycle readings during (and after) the reading process.
Decay correction on tube reading

Validations check during tube readings
Stingray can inform of failed validations before a tube readings is completed (where a single tube reader is being used).

Interleaved tube readings
Read a single tube at different parameters, before reading subsequent tubes. Raw data will be placed in separate matrices.

View Cut-Offs
For individual wells or by defined groups.

3D Graphs
Stingray can plot 3D graphs off raw or calculated data. Views can be zoomed, rotated and shifted. 3D graphs can be used to easily identify and flag anomalies

Manually Flagging Wells
Easily flag single wells or groups from various views, including standards graph. All flagged items are logged and can be included in the report.

Flag Individual Kinetic Points
Flag invalid kinetic points that will be ignored by kinetic reduction methods.

Sample Identifications
Specify sample identifications to be used in the report. Paste data from other applications with a single mouse click.

Flexible Standards Graph View
Zoom, flag points, flag defined groups, test the curve for unknown values and change the range to plot within.

Calculation Log
Details calculations, explains errors and tracks flagged items. The log can be included in the report and saved to a file.

Readings Notes
Specify notes regarding a specific protocol run - these notes are stored with the readings data and may be included in the report.

Data Exports
Export raw or calculated data to Excel or to any other application using Windows clipboard.

Graphic Exports
Copy Stingray graphs into the Windows clipboard with one mouse click for immediate use in other applications.

Archived Standards Data

Extract standards data from protocol runs for use in transformations of future protocols.

Interactive standards editor
Create or edit archived standards data visually and test unknowns.

Locale format specifiers
Uses format specifiers of current locales, eg (1.23 UK, 1,23 German or French)

Import raw data from LABCOM/LUMICOM
Re-analyse old data with Stingray.

User names and passwords
Use in conjunction with LabLock to restrict user access and rights. LabLock can also be used to log user activity.

Year 2000 compliant

Supports Devices
- Anthos Lucy 1.0 Microplate Luminometer/Photometer
- Anthos Lucy 2.0 Microplate Luminometer/Photometer
- Anthos 2010 Microplate Reader (Stingray allows the 2010 to be used for kinetics)
- Anthos HTII/HTIII Microplate Reader (Stingray overcomes the 40 cycle kinetic limitation by software emulation)
- Anthos 2001 Microplate Reader (Stingray overcomes the 40 cycle kinetic limitation by software emulation)
- Labtech Jade Tube Luminometer
- Bio-Orbit 1253 Tube Luminometer
- Bio-Orbit 1258 Galaxy Microplate Luminometer/Photometer
- Wallac Victor 2

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Dazdaq software supports the instruments listed here. Dazdaq does not supply or distribute these instruments, for further information about instrumentation contact your manufacturer or local instrument supplier.

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